FLO or Flo may refer to:

• in Florence, South Carolina:
  • Florence Regional Airport
  • Florence (Amtrak station)
• Fairtrade Labelling Organizations International
• Flo, an American comedy television series
• Homo floresiensis or "Flo," a skeleton of an extinct species of humanoid
• Flo, a chimpanzee that was featured in several books and documentaries
• FLO TV (Forward Link Only) mobile video
• Family Liaison Officer
• Flo, Norway, a village in Stryn, Norway
• Flo Parish, Sweden, a village in Sweden
• Nexus 7 (2013), an Android tablet by Google under the codename flo.

Flo is an American sitcom which aired on CBS from March 24, 1980 to July 21, 1981. The series was a spin-off of Alice starring Polly Holliday in the title role as the sassy and street-smart waitress Florence Jean "Flo" Castleberry. Flo was taped in front of a live audience at The Burbank Studios, Stage 6 in Burbank, California. Although the series was in the Top 20 throughout its run, it was cancelled at the end of its second season.