CART may refer to:

• Center for Advanced Research and Technology, a high tech high school located in Clovis, California
• Centre for Appropriate Rural Technology, South Africa
• Certified Anger Resolution Therapist, a certification for professional counselors
• Championship Auto Racing Teams, the former name of Champ Car, a now-defunct racing series
• Chimeric antigen receptor T cells.
• Christian African Relief Trust, an organisation dedicated to distributing aid based in West Yorkshire, UK
• Classification and regression tree, a type of decision tree learning, used in machine learning, data mining and predictive analytics
• Cleveland Area Rapid Transit
• Cocaine- and amphetamine-regulated transcript, a neuropeptide produced in the brain
• Coleshill Auxiliary Research Team, British historical research group
• Combination Antiretroviral Therapy, a type of treatment for HIV/AIDS
• Communication Access Real-Time Translation, a speech to text conversion system
• Computer Analysis Response Team, a Federal Bureau of Investigation working group
• Torulene dioxygenase, an enzyme

A cart is a vehicle designed for transport, using two wheels and normally pulled by one or a pair of draught animals. A handcart is pulled or pushed by one or more people. It is different from a dray or wagon, which is a heavy transport vehicle with four wheels and typically two or more horses, or a carriage, which is used exclusively for transporting humans.