Differential interference contrast (
DIC)
microscopy, also known as
Nomarski interference contrast (
NIC) or
Nomarski microscopy, is an
optical microscopy technique used to enhance the
contrast in unstained, transparent
samples. DIC works on the principle of
interferometry to gain information about the
optical path length of the sample, to see otherwise invisible features. A relatively complex optical system produces an image with the object appearing black to white on a grey background. This image is similar to that obtained by
phase contrast microscopy but without the bright diffraction halo. The technique was developed by
Georges Nomarski.