A chromosome (chromo- + -some) is a packaged and organized structure containing most of the DNA of a living organism. It is not usually found on its own, but rather is structured by being wrapped around protein complexes called nucleosomes, which consist of proteins called histones. The DNA in chromosomes is also associated with transcription (copying of genetic sequences) factors and several other macromolecules. During most of the duration of the Cell cycle, a chromosome consists of one long double-stranded DNA molecule (with associated proteins). During S phase, the chromosome gets replicated, resulting in an 'X'-shaped structure called a metaphase chromosome. Both the original and the newly copied DNA are now called chromatids. The two "sister" chromatids join together at a protein junction called a centromere. Chromosomes are normally visible under a light microscope only when the cell is undergoing mitosis (cell division). Even then, the full chromosome containing both joined sister chromatids becomes visible only during a sequence of mitosis known as metaphase (when chromosomes align together, attached to the mitotic spindle and prepare to divide). This DNA and its associated proteins and macromolecules is collectively known as chromatin, which is further packaged along with its associated molecules into a discrete structure called a nucleosome. Chromatin is present in most cells, with a few exceptions - erythrocytes for example. Occurring only in the nucleus of eukaryotic cells, chromatin composes the vast majority of all DNA, except for a small amount inherited maternally which is found in mitochondria. In prokaryotic cells, chromatin occurs free-floating in cytoplasm, as these cells lack organelles and a defined nucleus. Bacteria also lack histones. The main information-carrying macromolecule is a single piece of coiled double-stranded DNA, containing many genes, regulatory elements and other noncoding DNA. The DNA-bound macromolecules are proteins, which serve to package the DNA and control its functions. Chromosomes vary widely between different organisms. Some species such as certain bacteria also contain plasmids or other extrachromosomal DNA. These are circular structures in the cytoplasm which contain cellular DNA and play a role in horizontal gene transfer.